Journal: International immunopharmacology

Article Title: Foxp2 inhibits Th9 cell differentiation and attenuates allergic airway inflammation in a mouse model of ovalbumin-induced asthma.

doi: 10.1016/j.intimp.2022.109060

Figure Lengend Snippet: Fig. 1. Anti-IL-9 antibody inhibits Th9 cells differentiation in asthmatic mice. Forty BALB/c mice were randomly divided into four groups: control group (Control), OVA-induced asthma model group (OVA), mouse IgG injection treatment group (IgG) and anti-IL-9 mAb injection treatment group (anti-IL-9). Four hours after the last OVA stimulation, the mice were anesthetized and killed to collect bronchoalveolar lavage fluid (BALF) and lung tissue. A-B. The percentage of Th9 cells in BALF was evaluated by flow cytometry in each group. C-D. The percentage of Th9 cells in lung tissue was evaluated by flow cytometry in each group. E-F. The levels of IL-9 in BALF and lung tissue were measured by ELISA in each group. G-H. The mRNA expression of BATF and IRF4 in lung tissue were detected by RT-PCR. I-K. The protein expression of BATF and IRF4 in lung tissue were detected by western blot. **P < 0.01,***p < 0.001 vs Control group, #P < 0.05, ##P < 0.01,###p < 0.001 vs IgG group.

Article Snippet: Lung homogenates and BALF/cells supernatant were measured using a mouse IL-9 ELISA kit (Elabscience) according to the manufacturer’s instructions.

Techniques: Control, Injection, Flow Cytometry, Enzyme-linked Immunosorbent Assay, Expressing, Reverse Transcription Polymerase Chain Reaction, Western Blot

Journal: International immunopharmacology

Article Title: Foxp2 inhibits Th9 cell differentiation and attenuates allergic airway inflammation in a mouse model of ovalbumin-induced asthma.

doi: 10.1016/j.intimp.2022.109060

Figure Lengend Snippet: Fig. 3. Down regulation of Foxp2 expression in Th9 cells Spleen cells were isolated from normal mice and CD4+T cells were sorted by magnetic beads. IL-4, anti–IFN-ɣ and TGF-β were used to induce the differentiation of Th9 cells. After 4 days of induction, cells were collected. A.The levels of IL-9 in the supernatant of pre-induction (CD4+T) and post-induction (Th9) cells were were measured by ELISA. B-C. The mRNA expres sion of IL-9 and Foxp2 in pre-induction (CD4+T) and post-induction (Th9) cells were detected by RT-PCR in each group. D-E. The protein expression of IL-9 and Foxp2 in pre-induction (CD4+T) and post-induction (Th9) cells were analyzed by western blot in each group. **P < 0.01,***p < 0.001 vs CD4 + T cell group.

Article Snippet: Lung homogenates and BALF/cells supernatant were measured using a mouse IL-9 ELISA kit (Elabscience) according to the manufacturer’s instructions.

Techniques: Expressing, Isolation, Magnetic Beads, Enzyme-linked Immunosorbent Assay, Reverse Transcription Polymerase Chain Reaction, Western Blot

Journal: International immunopharmacology

Article Title: Foxp2 inhibits Th9 cell differentiation and attenuates allergic airway inflammation in a mouse model of ovalbumin-induced asthma.

doi: 10.1016/j.intimp.2022.109060

Figure Lengend Snippet: Fig. 4. Overexpression of Foxp2 inhibits Th9 cell differentiation in vitro Th9 cells were infected withFoxp2 overexpressed lentivirus (LV-Foxp2) and control lentivirus (LV-NC), respectively, and no infected group as control. A-B. The percentage of Th9 cells was evaluated by Flow cytometry. C. The levels of IL-9 in cell supernatant was measured by ELISA. D. The mRNA expression of IL-9 in cells was detected by RT-PCR. E-F. The mRNA expression of BATF and IRF4 in cells were detected by RT- PCR. **P < 0.01, ***p < 0.001 vs LV-NC group.

Article Snippet: Lung homogenates and BALF/cells supernatant were measured using a mouse IL-9 ELISA kit (Elabscience) according to the manufacturer’s instructions.

Techniques: Over Expression, Cell Differentiation, In Vitro, Infection, Control, Flow Cytometry, Enzyme-linked Immunosorbent Assay, Expressing, Reverse Transcription Polymerase Chain Reaction

Journal: PLoS ONE

Article Title: Treg/IL-17 Ratio and Treg Differentiation in Patients with COPD

doi: 10.1371/journal.pone.0111044

Figure Lengend Snippet: The original values for the selected cytokines were quantitated in serum obtained from healthy nonsmokers (n = 20) and subjects with SCOPD (n = 23) and AECOPD (n = 21) by ELISA (A). To eliminate the mixed effects of inflammatory factors, the authors divided each original value by the arithmetic mean of a classical inflammatory marker, such as TNF-α (B) and IL-17 (C). The data are presented as the mean ± SEM, unless otherwise stated. *p<0.05 and **p<0.01.

Article Snippet: The concentrations of cytokines (TGF-β, IL-10, TNF-α, IL-17 and IL-9) in serum were measured using ELISA kits according to the manufacturer’s protocols (all kits were purchased from Boster Biosciences Co., Wuhan, China).

Techniques: Enzyme-linked Immunosorbent Assay, Marker

Journal: Central-European journal of immunology

Article Title: Targeting the serum marker interleukin 9 improves the underlying characterization and immune homeostasis in rheumatoid arthritis.

doi: 10.5114/ceji.2024.141695

Figure Lengend Snippet: Fig. 3. Interleukin 9 affected the severity of arthritis in CIA rats. A) ELISA for the detection of IL-9 levels. B) Rat arthritis score. C) Analysis of cartilage damage by saffron O-solid green staining. The descriptive statistics are pre- sented as mean ±SD. *p < 0.05 vs. the CIA group, n = 3

Article Snippet: We measured the levels of serum markers using ELISA with a specialized commercial human IL-9 ELISA Kit (CSB-E04642h, Cusabio, Wuhan, China), human Gal1 ELISA Kit (CSB-EL012882h, Cusabio), human anti-CII ELISA Kit (HB3371-Hu, Shanghai Hengyuan Biotechnology Co., Ltd, Shanghai, China), human sSR-A ELISA Kit (HB3370-Hu, Shanghai Hengyuan Biotechnology Co., Ltd), human anti-Sa ELISA Kit (ml382511V), human RF ELISA Kit (ml060678V), human ACPA ELISA Kit (ml233305V), rat IL-9 ELISA Kit (CSB-E07294r, Cusabio), rat Gal1 ELISA Kit (JL25762, Shanghai Jianglai Biotechnology Co., Ltd, Shanghai, China), rat anti-CII ELISA Kit (HB1364-Ra, Shanghai Hengyuan Biotechnology Co., Ltd), rat sSR-A ELISA Kit (HB1363-Ra, Shanghai Hengyuan Biotechnology Co., Ltd), rat anti-Sa ELISA Kit (ml202241V), rat RF ELISA Kit (ml059541V), and rat ACPA ELISA Kit (ml201458V).

Techniques: Enzyme-linked Immunosorbent Assay, Staining

Journal: Central-European journal of immunology

Article Title: Targeting the serum marker interleukin 9 improves the underlying characterization and immune homeostasis in rheumatoid arthritis.

doi: 10.5114/ceji.2024.141695

Figure Lengend Snippet: Fig. 3. Interleukin 9 affected the severity of arthritis in CIA rats. A) ELISA for the detection of IL-9 levels. B) Rat arthritis score. C) Analysis of cartilage damage by saffron O-solid green staining. The descriptive statistics are pre- sented as mean ±SD. *p < 0.05 vs. the CIA group, n = 3

Article Snippet: We measured the levels of serum markers using ELISA with a specialized commercial human IL-9 ELISA Kit (CSB-E04642h, Cusabio, Wuhan, China), human Gal1 ELISA Kit (CSB-EL012882h, Cusabio), human anti-CII ELISA Kit (HB3371-Hu, Shanghai Hengyuan Biotechnology Co., Ltd, Shanghai, China), human sSR-A ELISA Kit (HB3370-Hu, Shanghai Hengyuan Biotechnology Co., Ltd), human anti-Sa ELISA Kit (ml382511V), human RF ELISA Kit (ml060678V), human ACPA ELISA Kit (ml233305V), rat IL-9 ELISA Kit (CSB-E07294r, Cusabio), rat Gal1 ELISA Kit (JL25762, Shanghai Jianglai Biotechnology Co., Ltd, Shanghai, China), rat anti-CII ELISA Kit (HB1364-Ra, Shanghai Hengyuan Biotechnology Co., Ltd), rat sSR-A ELISA Kit (HB1363-Ra, Shanghai Hengyuan Biotechnology Co., Ltd), rat anti-Sa ELISA Kit (ml202241V), rat RF ELISA Kit (ml059541V), and rat ACPA ELISA Kit (ml201458V).

Techniques: Enzyme-linked Immunosorbent Assay, Staining